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Troubleshooting PCR Services: A Practical Guide to Common Issues and Solutions
The polymerase chain reaction (PCR) is one of the most common techniques employed in molecular and medicinal biology. PCR includes replicating a single-stranded DNA using DNA polymerase and synthetic primers. Since its development in 1983, PCR has become a critical component of molecular biology with applications ranging from disease diagnosis to basic research, forensic investigation,…